Mini-course on SDS-PAGE

This Mini-Course on SDS-PAGE is your quick-start guide to mastering Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)-a core technique used for separating proteins based on size. Commonly applied in biochemistry, molecular biology, immunology, and pharmaceutical research, SDS-PAGE is essential for anyone working with protein samples. Whether you're a student learning lab techniques for the first time, a researcher refreshing your skills, or a technician supporting experimental workflows, this course delivers everything you need to know in a concise and practical format.

You'll begin with a clear introduction to the principles and history of SDS-PAGE, followed by a step-by-step guide to gel and sample preparation, electrophoresis setup, and gel staining. Visual demonstrations help you understand each stage of the process, along with valuable tips for optimizing your results. We'll also explore applications of SDS-PAGE, common issues and troubleshooting strategies, and introduce related techniques like Western blotting for further protein analysis.

Designed to be short but content-rich, this course helps you gain practical lab knowledge without spending hours on theory. The goal is to get you ready to perform SDS-PAGE confidently in any laboratory setting, following correct scientific procedures. By the end of this course, you'll have a strong understanding of how SDS-PAGE works and how to apply it effectively in your experiments.

If you're looking for a focused, easy-to-follow, and scientifically accurate course, you're in the right place.