Molecular Biology and biotechnology principles

In this course students will learn about molecular biology and biotechnology principles. 1. Unit 1Topics covered in: Lecture 1 , 2 and 3Recombinant DNA technology Tools used in Recombinant DNA technologyIsolation of vector gene Isolation of desired geneMethods to transfer foreign gene into bacterial cellDiscuss about Restriction enzymesAnd about ligases Vectors like cosmid, plasmid Production of insulin gene Applications of recombinant DNA technology2.

Unit 2Topics covered in Lecture 4 Molecular biology TechniquesSouthern blotting technique used for DNA samplesNorthern blotting used for RNA samples 3. Unit 3Topics covered in Lecture 5Hybridisation technique Polymerase chain reaction used to amplify the DNA in the sample Radio immuno Assay - Antigen and antibody specific Electrophoresis zone electrophoresis Gel electrophoresis Polyacrylamide gel electrophoresis. Paper electrophoresis.

Principle of electrophoresis. Used to separate the DNA depending on the size and charge on the molecule. Gel electrophoresis - is used to separate DNA or RNA.

SDS page used to separate proteins and amino acids4. Unit 4Topics covered in Lecture 6 and 7 chromatographycolumn chromatography Affinity chromatography. - based on affinity towards ligand and protein.